recombinant mouse il 17 receptor c il 17rc Search Results


recombinant mouse il 17 receptor c  (R&D Systems)
93
R&D Systems recombinant mouse il 17 receptor c
Recombinant Mouse Il 17 Receptor C, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il 17rc receptor  (R&D Systems)
93
R&D Systems human il 17rc receptor
Fig. 2. Confirmation of IL-4 Contamination in Recombinant IL-17F (PeproTech). (A) Cultured human fibroblasts were stimulated with IL-17A, IL-17F from both PeproTech and R&D Systems, and IL-4 (positive control) for 24 h to compare gene expression via qPCR (n = 6). The analysis initially aimed to compare IL-17A and IL- 17F, but unexpectedly, IL-17F from PeproTech induced IL-4-responsive genes (IL4R and CCL26). (B) Further tests with IL-17F from R&D Systems confirmed that pure IL-17F does not induce IL-4 genes. Data are presented as fold changes, normalized to RPLP0. Statistical significance: *p < 0.05, **p ≤0.01, ***p ≤0.001, ***p ≤ 0.0001, ns (not significant). (C) Cultured human fibroblasts were stimulated with recombinant IL-17F from PeproTech and R&D Systems for 15 min (n = 4). Western blot analysis shows STAT6 phosphorylation in cells treated with IL-17F from PeproTech, but not from R&D Systems. β-actin was used as the loading control. (D) Western blot analysis was performed to assess the presence of IL-4 in Recombinant Human IL-17F (Catalog# 200–25, Lot# 0312277) from PeproTech, purchased at two different time points. IL-4 was detected in the PeproTech IL-17F protein, while no IL-4 was present in the IL-17F from R&D Systems. These results confirm IL-4 contamination in the PeproTech recombinant IL-17F, which may misdescribe prior experimental findings attributed to IL-17F. For analysis, 0.5 μg of recombinant IL- 4 (PeproTech) was used as a positive control, along with 1 μg of Recombinant Human IL-17F from both PeproTech and R&D Systems. (E) Fibroblasts were stimulated with IL-17F (PeproTech) for 15 min. Antibodies against IL-17RA and <t>IL-17RC</t> were used to block IL-17 signaling. Mouse IgG1 kappa isotype served as a control. β-actin was the loading control.
Human Il 17rc Receptor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il+17+receptor+c+il+17rc/Human+IL-17RC+Antibody/pm39675101-48-22-29
Average 93 stars, based on 1 article reviews
human il 17rc receptor - by Bioz Stars, 2026-10
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polyclonal anti mouse il 17r carboxyfluorescein  (R&D Systems)
94
R&D Systems polyclonal anti mouse il 17r carboxyfluorescein
Fig. 2. Confirmation of IL-4 Contamination in Recombinant IL-17F (PeproTech). (A) Cultured human fibroblasts were stimulated with IL-17A, IL-17F from both PeproTech and R&D Systems, and IL-4 (positive control) for 24 h to compare gene expression via qPCR (n = 6). The analysis initially aimed to compare IL-17A and IL- 17F, but unexpectedly, IL-17F from PeproTech induced IL-4-responsive genes (IL4R and CCL26). (B) Further tests with IL-17F from R&D Systems confirmed that pure IL-17F does not induce IL-4 genes. Data are presented as fold changes, normalized to RPLP0. Statistical significance: *p < 0.05, **p ≤0.01, ***p ≤0.001, ***p ≤ 0.0001, ns (not significant). (C) Cultured human fibroblasts were stimulated with recombinant IL-17F from PeproTech and R&D Systems for 15 min (n = 4). Western blot analysis shows STAT6 phosphorylation in cells treated with IL-17F from PeproTech, but not from R&D Systems. β-actin was used as the loading control. (D) Western blot analysis was performed to assess the presence of IL-4 in Recombinant Human IL-17F (Catalog# 200–25, Lot# 0312277) from PeproTech, purchased at two different time points. IL-4 was detected in the PeproTech IL-17F protein, while no IL-4 was present in the IL-17F from R&D Systems. These results confirm IL-4 contamination in the PeproTech recombinant IL-17F, which may misdescribe prior experimental findings attributed to IL-17F. For analysis, 0.5 μg of recombinant IL- 4 (PeproTech) was used as a positive control, along with 1 μg of Recombinant Human IL-17F from both PeproTech and R&D Systems. (E) Fibroblasts were stimulated with IL-17F (PeproTech) for 15 min. Antibodies against IL-17RA and <t>IL-17RC</t> were used to block IL-17 signaling. Mouse IgG1 kappa isotype served as a control. β-actin was the loading control.
Polyclonal Anti Mouse Il 17r Carboxyfluorescein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il+17+receptor+c+il+17rc/Mouse+IL-17RC+Antibody/pmc02689857-89-0-3
Average 94 stars, based on 1 article reviews
polyclonal anti mouse il 17r carboxyfluorescein - by Bioz Stars, 2026-10
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il 17r  (R&D Systems)
90
R&D Systems il 17r
Fig. 2. Confirmation of IL-4 Contamination in Recombinant IL-17F (PeproTech). (A) Cultured human fibroblasts were stimulated with IL-17A, IL-17F from both PeproTech and R&D Systems, and IL-4 (positive control) for 24 h to compare gene expression via qPCR (n = 6). The analysis initially aimed to compare IL-17A and IL- 17F, but unexpectedly, IL-17F from PeproTech induced IL-4-responsive genes (IL4R and CCL26). (B) Further tests with IL-17F from R&D Systems confirmed that pure IL-17F does not induce IL-4 genes. Data are presented as fold changes, normalized to RPLP0. Statistical significance: *p < 0.05, **p ≤0.01, ***p ≤0.001, ***p ≤ 0.0001, ns (not significant). (C) Cultured human fibroblasts were stimulated with recombinant IL-17F from PeproTech and R&D Systems for 15 min (n = 4). Western blot analysis shows STAT6 phosphorylation in cells treated with IL-17F from PeproTech, but not from R&D Systems. β-actin was used as the loading control. (D) Western blot analysis was performed to assess the presence of IL-4 in Recombinant Human IL-17F (Catalog# 200–25, Lot# 0312277) from PeproTech, purchased at two different time points. IL-4 was detected in the PeproTech IL-17F protein, while no IL-4 was present in the IL-17F from R&D Systems. These results confirm IL-4 contamination in the PeproTech recombinant IL-17F, which may misdescribe prior experimental findings attributed to IL-17F. For analysis, 0.5 μg of recombinant IL- 4 (PeproTech) was used as a positive control, along with 1 μg of Recombinant Human IL-17F from both PeproTech and R&D Systems. (E) Fibroblasts were stimulated with IL-17F (PeproTech) for 15 min. Antibodies against IL-17RA and <t>IL-17RC</t> were used to block IL-17 signaling. Mouse IgG1 kappa isotype served as a control. β-actin was the loading control.
Il 17r, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aah blg 1000 4  (RayBiotech inc)
93
RayBiotech inc aah blg 1000 4
Fig. 2. Confirmation of IL-4 Contamination in Recombinant IL-17F (PeproTech). (A) Cultured human fibroblasts were stimulated with IL-17A, IL-17F from both PeproTech and R&D Systems, and IL-4 (positive control) for 24 h to compare gene expression via qPCR (n = 6). The analysis initially aimed to compare IL-17A and IL- 17F, but unexpectedly, IL-17F from PeproTech induced IL-4-responsive genes (IL4R and CCL26). (B) Further tests with IL-17F from R&D Systems confirmed that pure IL-17F does not induce IL-4 genes. Data are presented as fold changes, normalized to RPLP0. Statistical significance: *p < 0.05, **p ≤0.01, ***p ≤0.001, ***p ≤ 0.0001, ns (not significant). (C) Cultured human fibroblasts were stimulated with recombinant IL-17F from PeproTech and R&D Systems for 15 min (n = 4). Western blot analysis shows STAT6 phosphorylation in cells treated with IL-17F from PeproTech, but not from R&D Systems. β-actin was used as the loading control. (D) Western blot analysis was performed to assess the presence of IL-4 in Recombinant Human IL-17F (Catalog# 200–25, Lot# 0312277) from PeproTech, purchased at two different time points. IL-4 was detected in the PeproTech IL-17F protein, while no IL-4 was present in the IL-17F from R&D Systems. These results confirm IL-4 contamination in the PeproTech recombinant IL-17F, which may misdescribe prior experimental findings attributed to IL-17F. For analysis, 0.5 μg of recombinant IL- 4 (PeproTech) was used as a positive control, along with 1 μg of Recombinant Human IL-17F from both PeproTech and R&D Systems. (E) Fibroblasts were stimulated with IL-17F (PeproTech) for 15 min. Antibodies against IL-17RA and <t>IL-17RC</t> were used to block IL-17 signaling. Mouse IgG1 kappa isotype served as a control. β-actin was the loading control.
Aah Blg 1000 4, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il+17+receptor+c+il+17rc/Human+L1000+Array%2C+Glass+Slide/pm37769657-282-288-285
Average 93 stars, based on 1 article reviews
aah blg 1000 4 - by Bioz Stars, 2026-10
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il 17 receptor c il 17rc antibody  (R&D Systems)
90
R&D Systems il 17 receptor c il 17rc antibody
Fig. 2. Confirmation of IL-4 Contamination in Recombinant IL-17F (PeproTech). (A) Cultured human fibroblasts were stimulated with IL-17A, IL-17F from both PeproTech and R&D Systems, and IL-4 (positive control) for 24 h to compare gene expression via qPCR (n = 6). The analysis initially aimed to compare IL-17A and IL- 17F, but unexpectedly, IL-17F from PeproTech induced IL-4-responsive genes (IL4R and CCL26). (B) Further tests with IL-17F from R&D Systems confirmed that pure IL-17F does not induce IL-4 genes. Data are presented as fold changes, normalized to RPLP0. Statistical significance: *p < 0.05, **p ≤0.01, ***p ≤0.001, ***p ≤ 0.0001, ns (not significant). (C) Cultured human fibroblasts were stimulated with recombinant IL-17F from PeproTech and R&D Systems for 15 min (n = 4). Western blot analysis shows STAT6 phosphorylation in cells treated with IL-17F from PeproTech, but not from R&D Systems. β-actin was used as the loading control. (D) Western blot analysis was performed to assess the presence of IL-4 in Recombinant Human IL-17F (Catalog# 200–25, Lot# 0312277) from PeproTech, purchased at two different time points. IL-4 was detected in the PeproTech IL-17F protein, while no IL-4 was present in the IL-17F from R&D Systems. These results confirm IL-4 contamination in the PeproTech recombinant IL-17F, which may misdescribe prior experimental findings attributed to IL-17F. For analysis, 0.5 μg of recombinant IL- 4 (PeproTech) was used as a positive control, along with 1 μg of Recombinant Human IL-17F from both PeproTech and R&D Systems. (E) Fibroblasts were stimulated with IL-17F (PeproTech) for 15 min. Antibodies against IL-17RA and <t>IL-17RC</t> were used to block IL-17 signaling. Mouse IgG1 kappa isotype served as a control. β-actin was the loading control.
Il 17 Receptor C Il 17rc Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il+17+receptor+c+il+17rc/IL-17RC+Antibody/pm22019504-65-16-21
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il 17 receptor c il 17rc antibody - by Bioz Stars, 2026-10
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Fig. 2. Confirmation of IL-4 Contamination in Recombinant IL-17F (PeproTech). (A) Cultured human fibroblasts were stimulated with IL-17A, IL-17F from both PeproTech and R&D Systems, and IL-4 (positive control) for 24 h to compare gene expression via qPCR (n = 6). The analysis initially aimed to compare IL-17A and IL- 17F, but unexpectedly, IL-17F from PeproTech induced IL-4-responsive genes (IL4R and CCL26). (B) Further tests with IL-17F from R&D Systems confirmed that pure IL-17F does not induce IL-4 genes. Data are presented as fold changes, normalized to RPLP0. Statistical significance: *p < 0.05, **p ≤0.01, ***p ≤0.001, ***p ≤ 0.0001, ns (not significant). (C) Cultured human fibroblasts were stimulated with recombinant IL-17F from PeproTech and R&D Systems for 15 min (n = 4). Western blot analysis shows STAT6 phosphorylation in cells treated with IL-17F from PeproTech, but not from R&D Systems. β-actin was used as the loading control. (D) Western blot analysis was performed to assess the presence of IL-4 in Recombinant Human IL-17F (Catalog# 200–25, Lot# 0312277) from PeproTech, purchased at two different time points. IL-4 was detected in the PeproTech IL-17F protein, while no IL-4 was present in the IL-17F from R&D Systems. These results confirm IL-4 contamination in the PeproTech recombinant IL-17F, which may misdescribe prior experimental findings attributed to IL-17F. For analysis, 0.5 μg of recombinant IL- 4 (PeproTech) was used as a positive control, along with 1 μg of Recombinant Human IL-17F from both PeproTech and R&D Systems. (E) Fibroblasts were stimulated with IL-17F (PeproTech) for 15 min. Antibodies against IL-17RA and IL-17RC were used to block IL-17 signaling. Mouse IgG1 kappa isotype served as a control. β-actin was the loading control.

Journal: Cytokine

Article Title: When recombinant proteins go wrong: The hidden pitfall of recombinant protein contamination.

doi: 10.1016/j.cyto.2024.156830

Figure Lengend Snippet: Fig. 2. Confirmation of IL-4 Contamination in Recombinant IL-17F (PeproTech). (A) Cultured human fibroblasts were stimulated with IL-17A, IL-17F from both PeproTech and R&D Systems, and IL-4 (positive control) for 24 h to compare gene expression via qPCR (n = 6). The analysis initially aimed to compare IL-17A and IL- 17F, but unexpectedly, IL-17F from PeproTech induced IL-4-responsive genes (IL4R and CCL26). (B) Further tests with IL-17F from R&D Systems confirmed that pure IL-17F does not induce IL-4 genes. Data are presented as fold changes, normalized to RPLP0. Statistical significance: *p < 0.05, **p ≤0.01, ***p ≤0.001, ***p ≤ 0.0001, ns (not significant). (C) Cultured human fibroblasts were stimulated with recombinant IL-17F from PeproTech and R&D Systems for 15 min (n = 4). Western blot analysis shows STAT6 phosphorylation in cells treated with IL-17F from PeproTech, but not from R&D Systems. β-actin was used as the loading control. (D) Western blot analysis was performed to assess the presence of IL-4 in Recombinant Human IL-17F (Catalog# 200–25, Lot# 0312277) from PeproTech, purchased at two different time points. IL-4 was detected in the PeproTech IL-17F protein, while no IL-4 was present in the IL-17F from R&D Systems. These results confirm IL-4 contamination in the PeproTech recombinant IL-17F, which may misdescribe prior experimental findings attributed to IL-17F. For analysis, 0.5 μg of recombinant IL- 4 (PeproTech) was used as a positive control, along with 1 μg of Recombinant Human IL-17F from both PeproTech and R&D Systems. (E) Fibroblasts were stimulated with IL-17F (PeproTech) for 15 min. Antibodies against IL-17RA and IL-17RC were used to block IL-17 signaling. Mouse IgG1 kappa isotype served as a control. β-actin was the loading control.

Article Snippet: For experiments involving IL-17 receptor blockade, cultured fibroblasts were pre-incubated with antibodies against human IL-17RA receptor: Anti-hIL17RA (10 μg/mL; R&D Systems) and human IL- 17RC receptor: Anti-hIL17RC (10 μg/mL; R&D Systems).

Techniques: Recombinant, Cell Culture, Positive Control, Gene Expression, Western Blot, Phospho-proteomics, Control, Blocking Assay